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Series GSE87785 Query DataSets for GSE87785
Status Public on Jun 12, 2018
Title Sequencing-based analyses characterize a tumor suppressive role of mir-1271 repressed by DNA hypermethylation in gastric cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary To identify tumor suppressive microRNAs repressed by DNA hypermethylation in gastric cancer (GC), we analyzed methylome and miRNome of EpCAM+/CD44+ GC cells. Among a set of microRNAs hypermethylated and downregulated in GC, mir-1271 was uncovered as a microRNA repressed by DNA hypermethylation in GC. Forced expression of mir-1271 significantly suppressed growth, migration, and invasion of GC cells both in vitro and in vivo. To identify target genes and cancer signaling pathways regulated by mir-1271, we examined differentially-expressed genes responsive to mir-1271 by performing RNA-sequencing.
Overall design 2 paired normal and GC cancer cells miRNA-seq, 3 GC cell lines MBD-seq, miR-1271 mimic treated GC cells and control cells RNA-seq
Contributor(s) Lim B, Baek S, Kim J, Kim S, Kim YS, Kim M
Citation(s) 29862663
Submission date Oct 10, 2016
Last update date Jul 25, 2021
Contact name Jong Hwan Kim
Phone 0821093299557
Organization name Korea Research Institute of Bioscience and Biotechnology
Department Genome structure research center
Street address 125 Gwahak-ro, Yuseong-gu
City Daejeon
ZIP/Postal code ASI|KR|KS015|DAEJEON
Country South Korea
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (19)
GSM2340235 Normal2_miRNAseq
GSM2340236 csc2_miRNAseq
GSM2340237 Normal3_miRNAseq
BioProject PRJNA347596
SRA SRP091347

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87785_RAW.tar 89.1 Mb (http)(custom) TAR (of BEDGRAPH)
GSE87785_RNAseq_log2CPM.xlsx 2.3 Mb (ftp)(http) XLSX
GSE87785_miRNAseq_RPKM.xlsx 67.7 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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