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Status |
Public on Oct 09, 2019 |
Title |
VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development [ChIP-seq] |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
The number of vertebrae is precisely defined in almost all vertebrate species, but varies considerably in pigs, making this animal an excellent model for studying the mechanisms that control vertebral number. Vertnin (VRTN) variants have been associated with thoracic vertebral number (TVN) in pigs. However, the causal relation between VRTN and TVN remains to be established, and the role of VRTN in modulating TVN is not yet known. Here, we demonstrate that VRTN is one of the genes responsible for determining TVN. We show that VRTN is a DNA-binding transcription factor, which is essential for the formation of thoracic vertebrae during early embryogenesis as VRTN-null mice showed embryonic lethality at the later thoracic somite stages and had fewer somites than their wild-type and heterozygous littermates. We also show that VRTN causative variants increase Notch signaling in pig embryos, suggesting that VRTN controls segment number by altering the pace of somatic segmentation. These findings advance our understanding of the role of VRTN in the formation of thoracic vertebrae and reveal new aspects of somite developmental biology.
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Overall design |
Examination of VRTN binding sites in HEK293T cells transfected with pNTAP-2×flag-VRTN vector.
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Contributor(s) |
Duan Y, Zhang H, Zhang Z, Gao J, Yang J, Wu Z, Fan Y, Xing Y, Li L, Xiao S, Ren J, Huang L |
Citation missing |
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Submission date |
Oct 09, 2016 |
Last update date |
Oct 10, 2019 |
Contact name |
Yanyu Dean |
E-mail(s) |
yanyuduan@gmail.com
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Phone |
86-791-83813080
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Organization name |
Jiangxi Agricultural University
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Lab |
State Key Laboratory of Pig Genetic Improvement and Production Technology
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Street address |
Lushanzhong888, Changbei district
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City |
Nanchang |
ZIP/Postal code |
330044 |
Country |
China |
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Platforms (1) |
GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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Samples (1) |
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This SubSeries is part of SuperSeries: |
GSE87776 |
VRTN Regulates the Number of Thoracic Vertebrae During Vertebrate Development |
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Relations |
BioProject |
PRJNA347518 |
SRA |
SRP091308 |