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Series GSE87750 Query DataSets for GSE87750
Status Public on Jul 07, 2017
Title Zika virus directly infects human peripheral neurons and induces cell death
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Zika virus (ZIKV), a flavivirus transmitted primarily by Aedes mosquitoes, has spread to 59 countries and territories throughout the world. ZIKV-infected patients can have several symptoms and there is scientific consensus that ZIKV is a cause of microcephaly. Most studies are focusing on the central nervous system (CNS), and it is largely unknown whether ZIKV also affects the developing peripheral nervous system. Here, using a human pluripotent stem cell-based model, we demonstrated that Puerto Rican ZIKV strain, PRVABC59, efficiently infects human neural crest cells (hNCCs). In contrast to little infection of CNS neurons, ZIKV readily infects human peripheral neurons (hPNs), leading to cell death. Global gene expression analyses of infected hNCCs and hPNs reveal transcriptional dysregulation, notably of cell-death- and cell-cycle-related pathways. Our results identify hNCCs and hPNs as a direct ZIKV target and provide a framework to investigate the ZIKV pathology on the peripheral neurons.
Overall design Gene expression analyses of the cells in peripheral nervous system infected with the PRVABC59 strain of Zika virus
Contributor(s) Jin P
Citation(s) 28758997
Submission date Oct 07, 2016
Last update date May 15, 2019
Contact name Peng Jin
Phone 4047273729
Organization name Emory University
Department Human Genetics
Lab Jin Lab
Street address 615 Michael St., Rm 325
City Atlanta
State/province Georgia
ZIP/Postal code 30322
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (13)
GSM2339848 RNAseq-hPN-mock-1
GSM2339849 RNAseq-hPN-mock-2
GSM2339850 RNAseq-hPN-ZIKVPR-1
BioProject PRJNA347344
SRA SRP090990

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Supplementary file Size Download File type/resource
GSE87750_RAW.tar 5.6 Mb (http)(custom) TAR (of FPKM_TRACKING)
GSE87750_RNAseq-hNCC-ZIKVPR-1.fpkm_tracking.gz 815.6 Kb (ftp)(http) FPKM_TRACKING
GSE87750_RNAseq-hNCC-ZIKVPR-2.fpkm_tracking.gz 829.3 Kb (ftp)(http) FPKM_TRACKING
GSE87750_RNAseq-hNCC-mock-2.fpkm_tracking.gz 829.9 Kb (ftp)(http) FPKM_TRACKING
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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