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Series GSE87681 Query DataSets for GSE87681
Status Public on Nov 07, 2017
Title Analysis of gene expression from CD45neg cells of lung tumors upon neutrophil depletion or not
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Interrogating the tumor immune compartment generates new prognostic and treatment modalities. We used the poorly immunogenic oncogenic Kras driven mouse model of lung cancer, to challenge the predictive value of an immune signature, using a new algorithm called MegaClust. It highlighted a predominant negative impact of neutrophil infiltration on multiple immune populations. Human samples and depletion experiments in mice revealed that neutrophils promote tumor growth by altering angiogenesis, associated to increased PI3K/Akt/mTOR signaling in tumor cells and profound immunosubversion. Together with blood vessel spreading and normalization, neutrophil depletion was associated with T cell redistribution into tumor mass and sensitized tumors to anti-PD1 immunotherapy in this refractory model. Our observations illustrate a mechanism of immune exclusion protecting tumor cells from destruction that relies on neutrophil infiltration
Overall design Comparison of samples between anti-Ly6G and control IgG treatment
Contributor(s) Faget J, Zangger N, Meylan E
Citation(s) 29241546
Submission date Oct 06, 2016
Last update date May 15, 2019
Contact name Nadine Fournier
Organization name Agora Cancer Research Center & Swiss Institute of Bioinformatics
Lab Translational Data Science Facility
Street address Agora Cancer Research Center, Bugon 25A
City Lausanne
State/province Vaud
ZIP/Postal code 1000
Country Switzerland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2338498 anti-Ly6G Tri-1
GSM2338501 anti-Ly6G Tri-2
GSM2338503 anti-Ly6G Tri-3
BioProject PRJNA345547
SRA SRP090922

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87681_Ly6G_mouse_RNAseq_NormExpr_DESeq2.txt.gz 1.7 Mb (ftp)(http) TXT
GSE87681_Ly6G_mouse_RNAseq_RawCounts.txt.gz 772.2 Kb (ftp)(http) TXT
GSE87681_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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