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Series GSE87608 Query DataSets for GSE87608
Status Public on Sep 24, 2017
Title Next-Generation Sequencing Supports Quantitative Analysis of Wild Type and Runx2+/- Calvarial Transcriptomes With or Without Administration of MS-275
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Because insufficiency of the Runt-related transcription factor 2 (Runx2) limits skeletal growth, there is a great deal of effort to activate Runx2 for clinical use. In this study, we found that MS-275, the class I-specific HDAC inhibitor, activates Runx2 both transcriptionally and translationally. Therefore, we performed NGS analysis to gain accurate patterns of gene expression in mouse calvaria tissue through MS-275 administration. As a result, we could get insight that treatment of MS-275 increases genes related with osteoblast differentiation and cell proliferation, and decreases genes in field of causing apoptosis.
Overall design Mice calvarial mRNA profiles of embryonic day 17.5 wild type (WT) and Runx2+/- mice were generated by deep sequencing using Illumina NextSeq 500. Mice were administered MS-275 or vehicle. Three replicates per group.
Contributor(s) Bae H, Ryoo H
Citation(s) 28052439
Submission date Oct 04, 2016
Last update date May 15, 2019
Contact name Han-sol Bae
Organization name School of Dentistry, Seoul National University
Department Department of Molecular Genetics
Street address 1 Gwanak-ro, Gwanak-gu
City Seoul
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2335056 Mouse calvaria WT-vehicle 1
GSM2335057 Mouse calvaria WT-vehicle 2
GSM2335058 Mouse calvaria WT-vehicle 3
BioProject PRJNA345374
SRA SRP090822

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87608_RAW.tar 1.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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