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Series GSE87577 Query DataSets for GSE87577
Status Public on Mar 21, 2018
Title Transcriptional study of ARN8 cells treated with novel DHODH inhibitors
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We have previously observed protein level changes after treating human cells with two novel inhibitors of the enzyme DHODH. This study was designed to study up to what degree these protein differences can be also observed at level of mRNA. As part of the de novo pyrimidine synthesis pathway, DHODH inhibition is expected to decrease the levels of total RNA in cells. However, some effector proteins associated with the p53 pathway are increased after treatment with these inhibitors. This study aims to answer if the observed protein differences are based on an increased transcription or due to protein stabilization.
Overall design The data represent three independent experiments. In each experiment ARN8 cells were treated with two DHODH inhibitors (DrugA and DrugM) and a vehicle control.
Contributor(s) Popova G, Pelechano V, Lain S
Citation(s) 29549331
Submission date Oct 03, 2016
Last update date May 15, 2019
Contact name Vicent Pelechano
Organization name ScilifeLab - Karolinska Institutet
Department MTC
Street address Nobels väg 16
City Solna
ZIP/Postal code SE-17177
Country Sweden
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM2334700 DMS0-1_S1
GSM2334701 DMS0-2_S4
GSM2334702 DMS0-3_S7
BioProject PRJNA345223
SRA SRP090767

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Supplementary file Size Download File type/resource
GSE87577_count_table.txt.gz 643.2 Kb (ftp)(http) TXT
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