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Series GSE87552 Query DataSets for GSE87552
Status Public on Aug 29, 2018
Title Inhibition of endothelial Notch signaling attenuates inflammation [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Vascular endothelial cells upregulate the Notch ligand Jagged1 in response to inflammatory activation. Here we show that abrogation of endothelial Notch1 signaling impairs induction of key inflammatory target genes. Accordingly, inducible endothelial-targeted knockout of the canonical Notch transcription factor RBPJ reduces inflammation in a murine model of contact hypersensitivity, while overexpression of constitutive active Notch1 has the opposite effect. We show that the proinflammatory role of Notch1 can be attributed to direct genomic priming of the inflammatory landscape involving crosstalk with NF-κB/RelA and selective activation of enhancers associated with Notch-dependent inflammatory genes. This is the first evidence that canonical Notch signaling supports endothelial activation in response to inflammatory cytokines on the chromatin level.
Overall design Examination of Notch1, RBPJ, NF-κB/RelA, H3K27ac genomic localization by ChIP-seq, in unstimulated and ILβ1-stimulated human umbilical vein endothelial cells (HUVECs) with and without Notch1 knockdown
Contributor(s) la Cour Poulsen L, Jetne Edelmann R, Krüger S, Diegues-Hurtado R, Shah A, Espen Stav-Noraas T, Renzi A, Szymanska M, Wang J, Ehling M, Benedito R, Kasprzycka M, Bækkevold E, Sundnes O, Donaldson I, Midwood K, Scott H, Collas P, Siebel CW, Adams RH, Haraldsen G, Sundlisæter E, Hol J
Citation(s) 29449332
Submission date Oct 03, 2016
Last update date May 15, 2019
Contact name Guttorm Haraldsen
Organization name Oslo University Hospital
Department Department for Pathology
Street address Sognsvannsveien 20
State/province OSLO
ZIP/Postal code 0027
Country Norway
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (15)
GSM2333677 HUVEC_UT_Notch1
GSM2333679 HUVEC_Scr_RelA
This SubSeries is part of SuperSeries:
GSE85987 Inhibition of endothelial Notch signaling attenuates inflammation
BioProject PRJNA345175
SRA SRP090746

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Supplementary file Size Download File type/resource
GSE87552_RAW.tar 2.9 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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