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| Status |
Public on Sep 23, 2019 |
| Title |
Epigenetic modifications of gene promoters in the liver of Balb/c mice protected by vaccination against blood-stage malaria of Plasmodium chabaudi |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Protective vaccination induces survival of more than 80% of female Balb/c mice otherwise succumbing to blood-stage malaria of Plasmodium chabaudi. This study investigates the effect of protective vaccination and P. chabaudi infections on DNA methylation of gene promoters in the liver of Balb/c mice. Using DNA methylation microarrays, protective vaccination is shown to affect per se the constitutive DNA methylation status of numerous gene promoters in the liver: Promoters of 256 genes are hyper(=up)- and 345 genes are hypo (=down)-methylated at a significance of p<0.05. Gene enrichment analysis reveals that genes with down-methylated promoters are most statistically significant enriched (p<0.01) with functions related to negative regulation of transcription, whereas genes with up-methylated promoters are related to positive regulation of transcription and immune system. Infections with P. chabaudi also induce changes in promoter DNA methylation. In vaccinated mice, these changes are observable already on day 1 p.i. and, in particular, at peak parasitemia on day 8 p.i., when 571 and 1013 gene promoters have become up- and down-methylated, respectively, in relation to constitutive DNA methylation at a signicance level of p<0.05. The corresponding gene promoters in non-vaccinated mice are also responsive to infection, but by far not as pronounced as in vaccinated mice. Our data demonstrate that vaccination modifies DNA methylation of gene promoters in the liver and augments promoter responsiveness to infection-induced DNA methylation alterations, which ultimately control expression of those genes in the liver required for survival of otherwise lethal blood-stage malaria of P. chabaudi.
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| Overall design |
18 samples were analyzed;
Nd0, Non-vaccinated day 0, 3 replicates
Vd0, Vaccinated day 0, 3 replicates
Nd1, Non-vaccinated day 1, 3 replicates
Vd1, Vaccinated day 1, 3 replicates
Nd8, Non-vaccinated day 8, 3 replicates
Vd8, Vaccinated day 8, 3 replicates
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| Contributor(s) |
Al-Quraishy S, Dkhil M, Abdel-Baki AA, Ghanjati F, Erichsen L, Santourlidis S, Wunderlich F, Araúzo-Bravo MJ |
| Citation missing |
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| Submission date |
Sep 26, 2016 |
| Last update date |
Jan 15, 2022 |
| Contact name |
Marcos J. Araúzo-Bravo |
| E-mail(s) |
mararabra@yahoo.co.uk
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| Phone |
+34 943 00 6108
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| Organization name |
Max Planck Institute for Molecular Biomedicine
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| Department |
Cell and Developmental Biology
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| Lab |
Computational Biology and Bionformatics
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| Street address |
Rogentstrasse
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| City |
Muenster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL10324 |
Agilent-015279 Mouse CpG Island ChIP-on-Chip Microarray 2x105K (Probe Name version) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA344507 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87373_RAW.tar |
175.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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