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Series GSE87341 Query DataSets for GSE87341
Status Public on Dec 06, 2016
Title In Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The in vitro derivation and propagation of spermatogonial stem cells (SSCs) from pluripotent stem cells (PSCs) is a key goal in reproductive science. We show here that when aggregated with embryonic testicular somatic cells (reconstituted testes), primordial germ cell-like cells (PGCLCs) induced from mouse embryonic stem cells differentiate into spermatogonia-like cells in vitro and are expandable as cells that resemble germline stem cells (GSCs), a primary cell line with SSC activity. Remarkably, GSC-like cells (GSCLCs), but not PGCLCs, colonize adult testes and, albeit less effectively than GSCs, contribute to spermatogenesis and fertile offspring. Whole-genome analyses reveal that GSCLCs exhibit aberrant methylation at vulnerable regulatory elements, including those critical for spermatogenesis, which may restrain their spermatogenic potential. Our study establishes a strategy for the in vitro derivation of SSC activity from PSCs, which, we propose, relies on faithful epigenomic regulation.
 
Overall design Transcriptome analysis of mouse GSCs and GSCLCs using SC3-seq technology
 
Contributor(s) Ishikura Y, Yabuta Y, Ohta H, Hayashi K, Nakamura T, Okamoto I, Yamamoto T, Kurimoto K, Shirane K, Sasaki H, Saitou M
Citation(s) 27926879
Submission date Sep 26, 2016
Last update date May 15, 2019
Contact name Yukihiro Yabuta
E-mail(s) yabyab@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (108)
GSM2328360 GSC1_MS094_065
GSM2328361 GSC1_MS094_067
GSM2328362 GSC1_MS094_068
Relations
BioProject PRJNA344462
SRA SRP090461

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87341_expression_RPM_ishikura_v1.txt.gz 4.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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