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Series GSE87218 Query DataSets for GSE87218
Status Public on Nov 18, 2016
Title Epigenome maps of time-resolved monocyte to macrophage differentiation and innate immune memory (ATAC-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as LPS. We apply an integrated epigenomic approach to characterize the molecular events involved in LPS-induced tolerance in a time dependent manner. ChIP-seq, RNA-seq, WGBS and ATAC-seq data were generated. This analysis identified epigenetic programs in tolerance and trained macrophages, and the potential transcription factors involved.
Overall design Time-course in vitro culture of human monocytes. Two innate immune memory states can be induced in culture through an initial exposure of primary human monocytes to either LPS or BG for 24 hours, followed by removal of stimulus and differentiation to macrophages for an additional 5 days. Cells were collected at baseline (day 0), 1 hour, 4 hour, 24 hour and 6 days.
Contributor(s) Novakovic B, Habibi E, Wang S, Stunnenberg H
Citation(s) 27863248
Submission date Sep 22, 2016
Last update date May 15, 2019
Contact name Hendrik G Stunnenberg
Phone +31 (0)24 3610524
Organization name Radboud University
Department Molecular Biology
Street address Geert Grooteplein 28
City Nijmegen
ZIP/Postal code 6525 GA
Country Netherlands
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (13)
GSM2325679 ATAC_BG_1h_8447
GSM2325680 ATAC_BG_4h_8450
GSM2325681 ATAC_BG_d1_8453
This SubSeries is part of SuperSeries:
GSE85246 Epigenome maps of time-resolved monocyte to macrophage differentiation and innate immune memory
BioProject PRJNA343921
SRA SRP090300

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87218_RAW.tar 6.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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