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| Status |
Public on Sep 21, 2017 |
| Title |
Genome-wide expression patterns of microRNAs in ground state embryonic stem cells |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We apply deep small-RNA sequencing technology for high-throughput profiling of microRNAs in ground state embryonic stem cells (ESCs). We provide global expression signatures of microRNAs in ESCs cultured under serum, 2i, and R2i conditions. We report that microRNAs are significantly differentially expressed when ESCs are cultured under different conditions, and that ground state pluripotency features a uniqure microRNA signature which is mainly encoded by microRNA-coding sequences within the developmentally important DLK1-Dio3 locus. Finally, we indicate that microRNA upregulated in ground state pluripotent cells (i.e. 2i/R2i) contribute to the maintenace of ground state pluripotency through stimulating self-renewal and inhibiting multi-lineague differentiation.
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| Overall design |
Comprehensive expression analysis of microRNAs in serum, 2i, and R2i mouse ESCs using small RNA sequencing.
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| Contributor(s) |
Moradi S, Sharif-Zarchi A, Mollamohammadi S, Stubenvoll A, Günther S, Hosseini-Salekdeh G, Asgari S, Braun T, Baharvand H, Ahmadi A |
| Citation(s) |
29129685 |
| BioProject |
PRJNA343212 |
| |
| Submission date |
Sep 21, 2016 |
| Last update date |
Jun 11, 2019 |
| Contact name |
Ali Sharifi-Zarchi |
| E-mail(s) |
asharifiz@gmail.com
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| Organization name |
Royan Institute for Stem Cell Biology and Technology
|
| Street address |
Royan Institute, Bani Hashem Sq.
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| City |
Tehran |
| ZIP/Postal code |
P.O. Box: 16635-148 |
| Country |
Iran |
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| Platforms (1) |
| GPL22473 |
Illumina Genome Analyzer Iix (Mus musculus) |
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| Samples (6)
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| Relations |
| SRA |
SRP090002 |
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