NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE8717 Query DataSets for GSE8717
Status Public on Aug 08, 2007
Title Expression data from Human MPNST cancer cells infected with G207 and oncolytic HSV
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used microarrays to identify genes regulated during oncolytic HSV infection. Oncolytic herpes simplex viruses (oHSV) are promising anticancer therapeutics. We sought to identify alterations in gene expression during oHSV infection of human cancer cells. Human malignant peripheral nerve sheath tumor (MPNST) cells were infected with G207, an ICP34.5-deleted oHSV previously evaluated in clinical trials. G207-infected cells demonstrated massive degradation of cellular mRNAs, while a subset were upregulated. A gene signature of 21 oHSV-induced genes contained 7 genes known to be HSV-induced. Go ontology classification revealed that a majority of upregulated genes are involved in Jak/STAT signaling, transcriptional regulation, nucleic acid metabolism, protein synthesis and apoptosis. Ingenuity-defined functional networks highlighted nodes for AP-1 subunits and interferon signaling via STAT1, SOCS1, SOCS3 and RANTES. Upregulation of SOCS1 correlated with sensitivity of MPNST lines to G207 and depletion of SOCS1 reduced virus replication >1-log. The transcriptome of oHSV-induced genes may predict oncolytic efficacy and provides rationale for next generation oncolytics.
Keywords: treated vs non treated
 
Overall design 5 human MPNST cancer cell lines were infected with G207 or mock infected for 6 hours followed by RNA extraction and hybridization on Affymetrix microarrays.
 
Contributor(s) Mahller YY, Sakthivel B, Baird WH, Aronow BJ, Hsu Y, Cripe TP, Mehrian-Shai R
Citation(s) 18551144
Submission date Aug 07, 2007
Last update date Mar 25, 2019
Contact name Bruce J Aronow
E-mail(s) bruce.aronow@chmcc.org
Phone 513-636-4865
Organization name Cincinnati Children's Hospital Medical Center
Street address
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (22)
GSM215800 8814_Mock_1
GSM215801 90-8_Infect_1
GSM215802 90-8_Mock_2
Relations
BioProject PRJNA101961

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8717_MIAMI_CheckList.pdf 30.7 Kb (ftp)(http) PDF
GSE8717_RAW.tar 107.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap