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Series GSE86955 Query DataSets for GSE86955
Status Public on Sep 14, 2017
Title Newborn and young adult (2 months) mouse dorsal skin cell culture time-series RNA-Seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Organoid formation is critical to the progress of regenerative medicine. We use the formation of skin organoids from dissociated cells to study the self-organization process. Here we live-image the process which reveals an unexpectedly complex morphogenetic process: dissociated cells – aggregates – polarized cysts – fused cysts– planar skin – hair placodes. Transcriptome profiling and functional testing show orderly expression peaks of adhesion molecules, insulin-like growth factors, Wnts, extracellular matrix molecules, and matrix metalloproteinases are crucial to the sequential morphological transitions. We draw an analogy between these self-organizing morphogenetic behaviours and the self-assembly of molecules in biophysics, with different morphological stages connected by phase transition-like switches. Adult cells, normally stalled as aggregates, can be restored to reform hairy skin by reactivation of the self-organization process with timely supply of molecular cues. Physical processes and molecular events may work in tandem to achieve organoid self-formation in tissue engineering and in vivo.
Overall design Comparison of mRNA profiles in newborn and young adult (about 2 months) mouse dorsal skin reconstitution cells at different culture time points
Contributor(s) Chuong C
Citation(s) 28798065, 37996466
Submission date Sep 15, 2016
Last update date Dec 08, 2023
Contact name Mingxing Lei
Phone 3234422845
Organization name University of Southern California
Street address 2011 Zonal Ave HMR 304A
City Los Angeles
State/province California
ZIP/Postal code 90033
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (22)
GSM2310965 Newborn_0hr_Chuong496
GSM2310966 Newborn_0hr_Chuong501
GSM2310967 Newborn_6hr_Chuong497
BioProject PRJNA343078
SRA SRP089917

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86955_RAW.tar 2.5 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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