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Series GSE86910 Query DataSets for GSE86910
Status Public on May 15, 2017
Title RNA-seq transcriptonal profiling in human primary adult erythroid progenitor cells (ProEs) after shRNA-mediated depletion of TFAM and PHB2 expression
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The developing erythroid cells require highly coordinated gene expression and metabolism. By comparing the proteomic and transcriptomic changes in human hematopoietic stem/progenitor cells (HSPCs) and lineage-committed erythroid progenitors (ProEs), and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. Two principal mitochondrial factors TFAM and PHB2 are tightly regulated at the protein level and indispensable for mitochondria and erythropoiesis. To determine the role of TFAM and PHB2 in mitochondrial function during erythroid development, we employed shRNA-mediated depeltion of TFAM and PHB2 expression in differentiating erythroid cells, and performed RNA-seq transcriptional profiling analysis.
Overall design Human primary adult erythroblasts were generated ex vivo from CD34+ hematopoietic stem/progenitor cells (HSPCs) using a serum-free two-phase liquid culture system. CD34+ HSPCs were transduced with lentiviruses containing shRNAs against TFAM, PHB2, or the non-targeting (shNT) control, selected and differentiated to proerythroblasts (ProEs). Cells were harvested at day 5 of erythroid differentiation and total RNA were extracted for RNA-seq analysis.
Contributor(s) Liu X, Zhang Y, Shao Z, Xu J
Citation(s) 28504707
Submission date Sep 14, 2016
Last update date May 15, 2019
Contact name Jian Xu
Phone 214-648-6125
Organization name UT Southwestern Medical Center
Department Children's Research Institute
Street address 6000 Harry Hines Blvd. NL12.138B
City Dallas
State/province TX
ZIP/Postal code 75235
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2310252 RNAseq_A5-ProE-shNT-rep1
GSM2310253 RNAseq_A5-ProE-shNT-rep2
GSM2310254 RNAseq_A5-ProE-shTFAM-rep1
This SubSeries is part of SuperSeries:
GSE86912 Quantitative Proteomic and Transcriptomic Analysis Reveals Post-Transcriptional Regulation of Mitochondrial Biogenesis during Erythropoiesis
BioProject PRJNA343063
SRA SRP089927

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Supplementary file Size Download File type/resource
GSE86910_RNA-seq_A5-ProE_RPKM.txt.gz 469.3 Kb (ftp)(http) TXT
GSE86910_RNA-seq_A5-ProE_read_count.txt.gz 224.3 Kb (ftp)(http) TXT
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