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Series GSE86909 Query DataSets for GSE86909
Status Public on Sep 14, 2017
Title NFIA regulates pancreatic cell fate and adult physiology through vesicle trafficking
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Intracellular trafficking is essential for proper cell signaling. In the pancreas, secretory cells rely on trafficking to regulate blood glucose and digestion. Pancreatic disorders reflect defects in function or development, evoking considerable interest in understanding the molecular genetics governing pancreatic organogenesis. Here, we show the transcription factor NFIA regulates trafficking in both the embryonic and adult pancreas, affecting both developmental cell fate decisions and adult physiology. NFIA deletion from pancreatic progenitors led to the development of more acinar cells and ducts and fewer endocrine cells, whereas ectopic NFIA promoted endocrine formation. We found that NFIA’s effects on trafficking influence endocrine/exocrine cell fate decisions through regulation of Notch. Adult NFIA-deficient mice develop diabetic phenotypes due to impaired insulin granule trafficking and defects in acinar zymogen secretion. This study shows how a single transcription factor, NFIA, thus exerts profound effects on both embryonic cell fate and adult physiology by regulating vesicle trafficking.
Overall design 2 control and 2 NFIA fl/fl; Pdx1-cre samples, from pooled embryonic litters at E17.5
Contributor(s) Scavuzzo MA, Chmieloweic J, Yang D, Borowiak M
Citation(s) 30590051
Submission date Sep 14, 2016
Last update date Jan 29, 2020
Contact name Malgorzata Borowiak
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
ZIP/Postal code 77030
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2310248 control 1
GSM2310249 control 2
GSM2310250 NFIA fl/fl; Pdx1-cre 1
BioProject PRJNA342946
SRA SRP089860

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Supplementary file Size Download File type/resource
GSE86909_control_vs_nfia.gene_exp.diff.gz 835.9 Kb (ftp)(http) DIFF
GSE86909_control_vs_nfia.isoform_exp.diff.gz 1.1 Mb (ftp)(http) DIFF
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Processed data are available on Series record
Raw data are available in SRA

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