GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE86907 Query DataSets for GSE86907
Status Public on Jul 27, 2017
Title A novel requirement for Drosha in maintenance of mammalian CG methylation
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Using a protein interaction screen, we identify the Microprocessor component Drosha as a novel Dnmt1-interactor. Drosha-deficient ES cells display genomic hypomethylation which is not accounted for by changes in the levels of Dnmt proteins. Both genetic and transfection studies show that Drosha stimulates Dnmt1 methyltransferase activity. We identify two transcripts that are specifically upregulated in Drosha but not Dicer-deficient ES cells. Regions within these transcripts predicted to form stem-loop structures are processed by Microprocessor and can inhibit DNMT1-mediated methylation in vitro. Our results highlight Drosha as a novel regulator of mammalian DNA methylation and we propose that Drosha-mediated processing of RNA is necessary to ensure full Dnmt1 activity. This adds to the Drosha repertoire of non-miRNA dependent functions as well as implicating RNA in regulating Dnmt1 activity and correct levels of genomic methylation.
Overall design Mapping of 5-methylcytosine in wild type and Drosha-deficient ES cells by MethylC-Seq and mapping of RNA in wild type and Drosha-deficient ES cells by RNA-Seq
Contributor(s) Schmitz RJ
Citation(s) 28934508, 28934503
Submission date Sep 14, 2016
Last update date May 15, 2019
Contact name Robert J Schmitz
Organization name University of Georgia
Department Genetics
Street address B416 Davison Life Sciences
City Athens
State/province GA
ZIP/Postal code 30602
Country USA
Platforms (2)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM2310232 WT, AB1 (129Sv/Ev) ES cells
GSM2310233 Drosha-deficient ES cells, derived from AB1 ES cells
GSM2310234 Dnmt1-deficient ES cells derived from J1 ES cells
BioProject PRJNA342937
SRA SRP089858

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86907_Ooi_lab_M_musculus_wt_and_drosha_long_cuffdiff_out.txt.gz 1.2 Mb (ftp)(http) TXT
GSE86907_Ooi_lab_M_musculus_wt_and_drosha_mRNA_cuffdiff_out.txt.gz 1.2 Mb (ftp)(http) TXT
GSE86907_Ooi_lab_M_musculus_wt_and_drosha_short_cuffdiff_out.txt.gz 973.3 Kb (ftp)(http) TXT
GSE86907_RAW.tar 11.1 Gb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap