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Series GSE86864 Query DataSets for GSE86864
Status Public on May 15, 2017
Title ChIP-seq analysis of H3K27ac in K562 cells with shRNA-mediated depeltion of TFAM expression
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The developing erythroid cells require highly coordinated gene expression and metabolism. By comparing the proteomic and transcriptomic changes in human hematopoietic stem/progenitor cells (HSPCs) and lineage-committed erythroid progenitors (ProEs), and uncover pathways related to mitochondrial biogenesis enhanced through post-transcriptional regulation. Two principal mitochondrial factors TFAM and PHB2 are tightly regulated at the protein level and indispensable for mitochondria and erythropoiesis. Depletion of TFAM in erythroid cells alters intracellular metabolism, leading to elevated histone acetylation, deregulated gene expression, defective mitochondria function and erythropoiesis.
Overall design To examine the global effects of histone hyperactylation in Tfam-deficient erythroid cells, we performed ChIP-seq analysis of H3K27ac in human K562 cells transduced with TFAM-specific or control (shNT) shRNAs.
Contributor(s) Liu X, Zhang Y, Shao Z, Xu J
Citation(s) 28504707
Submission date Sep 13, 2016
Last update date May 15, 2019
Contact name Jian Xu
Phone 214-648-6125
Organization name UT Southwestern Medical Center
Department Children's Research Institute
Street address 6000 Harry Hines Blvd. NL12.138B
City Dallas
State/province TX
ZIP/Postal code 75235
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM2309710 H3K27ac_K562_shNT
GSM2309711 H3K27ac_K562_TFAM-sh1
GSM2309712 H3K27ac_K562_TFAM-sh2
This SubSeries is part of SuperSeries:
GSE86912 Quantitative Proteomic and Transcriptomic Analysis Reveals Post-Transcriptional Regulation of Mitochondrial Biogenesis during Erythropoiesis
BioProject PRJNA342801
SRA SRP089799

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Supplementary file Size Download File type/resource
GSE86864_RAW.tar 789.2 Mb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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