GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE86857 Query DataSets for GSE86857
Status Public on Sep 17, 2016
Title A total RNA-Seq screen reveals that the splicing inhibitor Isoginkgetin blocks transcription elongation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Pharmacological perturbation is a powerful tool for understanding gene expression, but identification of the specific steps of this multi-step process targeted by small molecules remains challenging. Here we apply total RNA-Seq to distinguish specific pharmacological effects on transcription and pre-mRNA processing, revealing unexpectedly that the splicing inhibitor isoginkgetin blocks transcription elongation.An RNA-Seq screen reveals that the compounds TBBz and CYT387 mimic the transcriptional effects of isoginkgetin and are inhibitors of CSNK2A2, suggesting that isoginkgetin blocks transcription elongation via inhibition of CSNK2A2. Our results reveal RNA-Seq screening as a tool for disentangling complex pharmacological effects on gene expression.
Overall design Total RNA sequencing of drug treated Hela cells for 6 or 18 hours.
Contributor(s) Gray JM, Springer M, Boswell SA
Citation(s) 28263964
Submission date Sep 13, 2016
Last update date Jun 15, 2020
Contact name jesse M gray
Phone 6174321877
Organization name Harvard Medical School
Department Genetics
Lab Gray lab
Street address 77 avenue louis pasteur, NRB 356
City boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (80)
GSM2309438 D6-26_CPT
GSM2309439 D6-26_IsG
GSM2309440 D6-26_c10
BioProject PRJNA343235
SRA SRP091854

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86857_RAW.tar 34.1 Mb (http)(custom) TAR (of BEDGRAPH)
GSE86857_file.tar.gz 184.6 Mb (ftp)(http) TAR
GSE86857_readme.txt.gz 4.1 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap