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Series GSE86789 Query DataSets for GSE86789
Status Public on Sep 10, 2016
Title Differential responses of choroidal melanocytes and uveal melanoma cells to low oxygen conditions
Organism Homo sapiens
Experiment type Expression profiling by array
Summary PURPOSE: Tissue culture is traditionally performed at atmospheric oxygen concentration (21%), which induces hyperoxic stress, as endogenous physiologic oxygen tension found in tissues varies between 2% and 9%. This discrepancy may lead to misinterpretation of results and may explain why effects observed in vitro cannot always be reproduced in vivo and vice versa. Only a few studies have been conducted in low physiologic oxygen conditions to understand the development and differentiation of cells from the eye.
METHODS: The aim of this study was to investigate the growth and gene expression profile of melanocytes from the choroid permanently exposed to 21% (hyperoxic) or 3% (physiologic) oxygen with proliferation assays and DNA microarray. The cellular behavior of the melanocytes was then compared to that of cancer cells.
RESULTS: The gross morphology and melanin content of choroidal melanocytes changed slightly when they were exposed to 3% O2, and the doubling time was statistically significantly faster. There was an increase in the percentage of choroidal melanocytes in the active phases of the cell cycle as observed by using the proliferation marker Ki67. The caveolin-1 senescence marker was not increased in choroidal melanocytes or uveal melanoma cells grown in hyperoxia. In comparison, the morphology of the uveal melanoma cells was similar between the two oxygen levels, and the doubling time was slower at 3% O2. Surprisingly, gene expression profiling of the choroidal melanocytes did not reveal a large list of transcripts considerably dysregulated between the two oxygen concentrations; only the lactate transporter monocarboxylate transporter (MCT4) was statistically significantly upregulated at 3% O2.
CONCLUSIONS: This study showed that the oxygen concentration must be tightly controlled in experimental settings, because it influences the subsequent cellular behavior of human choroidal melanocytes.
Overall design Three primary cultures of choroidal melanocytes cultured at two percentages of oxygen are analyzed by gene profiling.
Web link
Contributor(s) Weidmann C, Pomerleau J, Trudel-Vandal L, Landreville S
Citation(s) 28356703
Submission date Sep 09, 2016
Last update date Nov 27, 2018
Contact name Solange Landreville
Organization name Université Laval
Department Ophthalmology
Street address 1050 chemin Sainte-Foy, Room H2-02
City Quebec
State/province Quebec
ZIP/Postal code G1S4L8
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM2308744 Choroidal_melanocytes_M1_21% oxygen_passage2_rep1
GSM2308745 Choroidal_melanocytes_M2_21% oxygen_passage2_rep1
GSM2308746 Choroidal_melanocytes_M3_21% oxygen_passage2_rep1
BioProject PRJNA342500

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86789_RAW.tar 78.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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