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| Status |
Public on Aug 20, 2007 |
| Title |
PPARg regulated gene expression in human dendritic cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In order to gain insights into how PPARg regulates different facets of dendritic cell (DC) differentiation, we sought to identify PPARg regulated genes and gene networks in monocyte-derived dendritic cells using global gene expression profiling. We employed an exogenous ligand activation approach using a selective PPARg ligand (rosiglitazone abbreviated as RSG). In addition, we have defined culture conditions in which human serum (HS) induces PPARg activation via a yet uncharacterized endogenous mechanism. We also compared the gene expression profile of developing dendritic cells obtained from patients harboring dominant negative mutations of the PPARg receptor (C114R and C131Y).
Keywords: ligand response
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| Overall design |
Monocytes were cultured for 6, 24 hours or 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF; cytokine treatment was repeated at day 3. Cells were obtained from 12 healthy individuals (6 biological replicates; the 6 and 24 hours samples were obtained from a single individual but the 5 days samples from a different one). Ligands were added at the beginning of differentiation. The 6 and 24 hours cells were treated with vehicle (DC) or 1 uM rosiglitazone (DC RSG), in the case of 5 day cultured cells 2.5 uM RSG was used. Cells were cultured in RPMI plus 10% FBS, in the case of DC4-DC6 cells were also cultured in human AB serum (DC HS). Finally we also obtained cells from patients harboring point mutations of the PPARg receptor (C114R and C131Y)
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| Contributor(s) |
Szatmari I, Nagy T, Agostini M, Chatterjee K, Nagy L |
| Citation(s) |
17664351, 20410489, 21632707 |
| Submission date |
Aug 02, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Istvan Szatmari |
| E-mail(s) |
szatmari@indi.biochem.dote.hu
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| Phone |
36 52 416-432
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| Fax |
36 52 314-989
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| Organization name |
University of Debrecen
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| Department |
Department of Biochemistry and Molecular Biology
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| Lab |
Nuclear Hormone Receptor
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| Street address |
Egyetem tér 1.
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| City |
Debrecen |
| ZIP/Postal code |
H-4010 |
| Country |
Hungary |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (63)
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| Relations |
| BioProject |
PRJNA101857 |
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