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Series GSE86467 Query DataSets for GSE86467
Status Public on Jan 20, 2017
Title Pervasive translational regulation of the cell Q1 signalling circuitry underlies mammalian development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The degree and dynamics of translational control during mammalian development remain poorly understood. Here we monitored translation of the mammalian genome as cells become specified and organize into tissues in vivo. This identified unexpected and pervasive translational regulation of most of the core signalling circuitry including Shh,Wnt, Hippo, PI3K and MAPK pathways. We further identify and functionally characterize a complex landscape of upstream open reading frames (uORFs) across 5'-untranslated regions (UTRs) of key signalling components. Focusing on the Shh pathway, we demonstrate the importance of uORFs within the major SHH receptor, Ptch1, in control of cell signalling and neuronal differentiation. Finally, we show that the expression of hundreds of mRNAs underlying critical tissue-specific developmental processes is largely regulated at the translation but not transcript levels. Altogether, this work reveals a new layer of translational control to major signalling components and gene regulatory networks that diversifies gene expression spatially across developing tissues.
Overall design Here, we monitored translation of the mammalian genome as cells become specified and organize into tissues in-vivo.
Contributor(s) Fujii K, Shi Z, Zhulyn O, Denans N, Barna M
Citation(s) 28195124
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 HD086634 5'UTR RNA Regulons in ribosome-mediated control of embryonic development STANFORD UNIVERSITY Barna
Submission date Sep 06, 2016
Last update date May 15, 2019
Contact name Zhen Shi
Organization name Stanford
Street address 279 Campus Drive
City Stanford
State/province California
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM2303104 Mesoderm_RPF 1
GSM2303105 Mesoderm_RPF 2
GSM2303106 Mesoderm_RPF 3
BioProject PRJNA342003
SRA SRP086704

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Supplementary file Size Download File type/resource
GSE86467_E11.5_NT_FL_normalizedReads.txt.gz 479.0 Kb (ftp)(http) TXT
GSE86467_E11.5_mesoderm_normalizedReads.txt.gz 278.9 Kb (ftp)(http) TXT
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