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Series GSE86205 Query DataSets for GSE86205
Status Public on Aug 30, 2016
Title Tissue-specific DamID profiling of chromatin proteins in Drosophila melanogaster
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Analysis of gene expression regulation typically requires identification of genomic sites where regulatory proteins bind. For this purpose, ChIP and DamID methods applied to cell lines or model organisms are now routinely used, even in selected cell types. In this work, we present modifications to experimental DamID protocol, as well as a custom data processing algorithm that allows to confidently identify genomic sites enriched with the proteins of interest. This algorithm is implemented in Perl and is also available as executable files thereby making DamID analysis relatively straightforward. Finally, we demonstrate how this pipeline performs when fed with real experimental data.
Overall design Examination of comr chromatin protein binding to chromosomes in wild type adult testes using DamID technique.
Contributor(s) Maksimov DA, Laktionov PP, Belyakin SN
Citation(s) 27766446
Submission date Aug 29, 2016
Last update date May 15, 2019
Contact name Daniil Maksimov
Organization name MCB NSC RAS
Lab Genomics lab
Street address Lavrentjeva ave., 8/2
City Novosibirsk
ZIP/Postal code 630090
Country Russia
Platforms (1)
GPL16479 Illumina MiSeq (Drosophila melanogaster)
Samples (4)
GSM2297726 Dam-1
GSM2297727 Dam-comr-1
GSM2297728 Dam-2
BioProject PRJNA340890
SRA SRP083316

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86205_Comr_WT_Testes_at_FDR_0.05_full_divided_track.wig.gz 1.7 Mb (ftp)(http) WIG
GSE86205_Comr_WT_Testes_at_FDR_0.05_probability_track.wig.gz 2.3 Mb (ftp)(http) WIG
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Raw data are available in SRA
Processed data are available on Series record

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