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Series GSE86168 Query DataSets for GSE86168
Status Public on Feb 09, 2017
Title Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation [h3k27 ChIP]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary IL-10 limits the magnitude of inflammatory gene expression following microbial stimuli and is essential to prevent inflammatory disease, however, the molecular basis for IL-10 mediated inhibition remains elusive. Using a genome-wide approach we demonstrate that inhibition of transcription is the primary mechanism for IL-10-mediated suppression in LPS-stimulated macrophages, and that inhibited genes can be divided into two clusters. Genes in the first cluster are inhibited only if IL-10 is included early in the course of LPS stimulation and is strongly enriched for interferon-inducible genes. Genes in the second cluster can be rapidly suppressed by IL-10 even after transcription is initiated, and this is associated with suppression of LPS-induced enhancer activation. Interestingly, the ability of IL-10 to rapidly suppress active transcription exhibits a delay following LPS stimulation. Thus, a key pathway for IL-10 mediated suppression involves rapid inhibition of enhancer function during the secondary phase of the response to LPS.
Overall design 3 independent macrophage pools were stimulated with LPS for 195 min, LPS and IL-10 for 195 min, or LPS for 195 min with the adddition of IL-10 for the last 30 min. ChIP-Seq performed for H3K27ac
Contributor(s) Conaway EA, de Oliveira DC, McInnis C, Snapper S, Horwitz BH
Citation(s) 28213503
Submission date Aug 29, 2016
Last update date May 15, 2019
Contact name Bruce Horwitz
Organization name Brigham and Women's Hospital
Department Pathology
Street address 77 Ave Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (13)
GSM2296665 H3K27ac untreated Rep1
GSM2296666 H3K27ac untreated Rep2
GSM2296667 H3K27ac untreated Rep3
This SubSeries is part of SuperSeries:
GSE86170 Inhibition of inflammatory gene transcription by IL-10 is associated with rapid suppression of LPS-induced enhancer activation
BioProject PRJNA340447
SRA SRP083192

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