|
| Status |
Public on Oct 30, 2008 |
| Title |
In vivo study of ADH-1 in combination with melphalan for treatment of melanoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To identify patterns of change in gene expression that correlated with drug treatment (saline control, ADH-1 alone, melphalan alone and ADH-1 plus melphalan) in our animal model of regional therapy for extremity melanoma we evaluated gene expression using microarray genechips and a Pearson correlation analysis. Genes were ranked according to the degree to which expression correlated with systemic ADH-1 treatment alone or in combination with regional melphalan. Tissue samples were harvested at 4, 24 and 48 hours after treatment with melphalan.
Specific cellular pathways associated with drug treatment were identified using gene set enrichment analysis (GSEA).
Keywords: time course; chemotherapy; melanoma; melphalan; N-cadherin; ADH-1
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| Overall design |
Sample size was 2 rats for each drug treatment group at both the 4 and 24 hour time points. At the 48 hour time point sample size was 1 rat for each drug treatment group. ADH-1 treatment preceded melphalan by 1 hour. ADH-1 treatment was repeated at 24 and 48 hrs. Tissue was harvested for RNA analysis at 4, 24 and 48 hours after completion of isolated limb infusion with melphalan (and 1 hour after an ADH-1 dose).
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| Contributor(s) |
Augustine CK, Tyler DS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 27, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Christina Augustine |
| E-mail(s) |
christi.augustine@duke.edu
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| Organization name |
Duke University Medical Center
|
| Department |
Surgery
|
| Street address |
VAMC RmE4001 508 Fulton St.
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27705 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (19)
|
| GSM213622 |
ADH-1 ip saline ILI 4hrs post ILI sample A |
| GSM213623 |
ADH-1 ip saline ILI 4hrs post ILI sample B |
| GSM213624 |
Saline ip saline ILI 4hrs post ILI sample A |
| GSM213625 |
Saline ip saline ILI 4hrs post ILI sample B |
| GSM213626 |
ADH-1 ip saline ILI 48hrs post ILI |
| GSM213627 |
ADH-1 ip LPAM ILI 48hrs post ILI |
| GSM213628 |
Saline ip LPAM ILI 48hrs post ILI |
| GSM213629 |
Saline ip saline ILI 48hrs post ILI |
| GSM213630 |
Saline ip saline ILI 24hrs post ILI sample A |
| GSM213632 |
Saline ip saline ILI 24hrs post ILI sample B |
| GSM213633 |
Saline ip LPAM ILI 24hrs post ILI sample A |
| GSM213634 |
Saline ip LPAM ILI 24hrs post ILI sample B |
| GSM213635 |
ADH-1 ip saline ILI 24hrs post ILI sample A |
| GSM213636 |
ADH-1 ip saline ILI 24hrs post ILI sample B |
| GSM213637 |
ADH-1 ip LPAM ILI 24hrs post ILI sample A |
| GSM213638 |
ADH-1 ip LPAM ILI 24hrs post ILI sample B |
| GSM213639 |
ADH-1 ip LPAM ILI 4hrs post ILI sample A |
| GSM213640 |
ADH-1 ip LPAM ILI 4hrs post ILI sample B |
| GSM213641 |
Saline ip LPAM ILI 4hrs post ILI |
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| Relations |
| BioProject |
PRJNA101791 |
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