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Series GSE86019 Query DataSets for GSE86019
Status Public on Dec 31, 2016
Title Dicer maintains the identity and function of proprioceptive sensory neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During development neurons achieve a high degree of specialization (Fishell and Heintz, 2013). Over time, while continuously recycling their components and despite transcriptional noise, their own respective properties remain intact. The transcription factors that play a large role in initially establishing neuronal identity can be required for maintaining it (Deneris and Hobert, 2014). Post-transcriptional regulation is also important to differentiation (Bian and Sun, 2011) but its role in maintaining cell identity is less established. To better understand how post-transcriptional regulation might contribute to cell identity, we examined the proprioceptive neurons in the dorsal root ganglion (DRG), a highly specialized sensory class, whose properties are well established and display clear differences when compared to other neurons in the ganglion. By conditionally ablating Dicer in mice, using a parvalbumin (Pvalb) driven cre, we impaired post-transcriptional regulation in the proprioceptive sensory neuron population. KO animals display a progressive form of ataxia at the beginning of the fourth postnatal week that is mirrored by a cell-death within the DRG. Before cell-loss, expression profiling shows a reduction of proprioceptor specific genes and an increased expression of non-proprioceptive genes normally enriched in other ganglion neurons. Furthermore, although central connections of these neurons are intact, the peripheral connections to the muscle are functionally impaired. Post-transcriptional regulation is therefore necessary to retain the transcriptional identity and support functional specialization of the proprioceptive sensory neurons.
Overall design mRNA levels were examined in the lumbar 4 dorsal root ganglion parvalbumin positive neurons with and without conditional ablation of dicer driven by parvalbumin-cre.
Contributor(s) O'Toole SM, Nelson SB
Citation(s) 28003412
Submission date Aug 24, 2016
Last update date May 15, 2019
Contact name Sean M O'Toole
Organization name FMI
Department Neuroscience
Lab Keller Lab
Street address Maulbeerstrasse 66
City Basel
State/province Basel-Stadt
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (6)
GSM2290686 WT_1
GSM2290687 WT_2
GSM2290688 WT_3
BioProject PRJNA339998

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Supplementary file Size Download File type/resource
GSE86019_pvalb_dicer_KO_TPM_values.csv.gz 336.3 Kb (ftp)(http) CSV
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Processed data are available on Series record
Raw data provided as supplementary file

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