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Series GSE85857 Query DataSets for GSE85857
Status Public on Apr 17, 2017
Title Runx1 stabilizes the mammary epithelial cell phenotype and prevents epithelial to mesenchymal transition
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The Runx1 transcription factor is essential for hematopoietic differentiation and mutations underlie various leukemias. Here we demonstrate a role for Runx1 in the MCF10 cell series model of breast cancer progression. The highest level of Runx1 that occurs in normal like mammary epithelial cells (MCF10A) is decreased in tumorigenic (MCF10AT1) and metastatic (MCF10CA1a) breast cancer cells. We show that depletion of Runx1 in MCF10A cells results in striking changes in cell morphology and induction of epithelial-mesenchymal transition (EMT) via several signaling pathways. Analyses of breast tumors and patient survival data reveal that loss of Runx1 is associated with poor prognosis and decreased survival. Re-expressing Runx1 in MCF10AT1 breast cancer cells restores the epithelial phenotype. These results identify a novel function for Runx1 in sustaining normal epithelial morphology and preventing EMT. These mechanisms suggest Runx1 levels in early stage tumors can be used as a prognostic indicator of tumor progression.
Overall design RNA-Seq; two replicates each sample (6 total)
Contributor(s) Hong D, Tye C, Boyd J, Lian J, Stein G
Citation(s) 28407681
Submission date Aug 19, 2016
Last update date May 15, 2019
Contact name Jonathan AR Gordon
Organization name University of Vermont
Department Biochemistry
Street address 89 Beaumont Ave Given E209
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (6)
GSM2285709 MCF10A_R1
GSM2285710 MCF10A_R2
GSM2285711 MCF10A_R3
BioProject PRJNA339558
SRA SRP082418

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85857_MCF10A_growthfactors_normalized_counts.txt.gz 577.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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