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Status |
Public on Aug 01, 2021 |
Title |
RNA-Chromatin Interactome Reveals ncRNA Functions for Transcription Regulation in 3D Genome Organization |
Organisms |
Drosophila melanogaster; Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing Other
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Summary |
Noncoding RNAs (ncRNAs) are an emerging class of regulatory molecules with a broad range of regulatory functions believed to be mediated by ncRNA-chromatin interactions. Genome-wide understanding of ncRNA functions requires precise mapping of all ncRNAs and their target loci. Current methods for studying chromatin-associated ncRNA lack specificity or are limited to singly assessing RNAs. We devised an unbiased strategy to identify all RNA Interactions with Chromatin by Paired-End-Taging (RICh-PET) and applied this approach to characterize the Drosophila RNA-chromatin interactome. We discovered that ncRNAs primarily target promoters and enhancers in open chromatin regions in colocalization with RNAPII and other TFs, suggesting combinatorial regulatory instructions for each locus. Enzymatic nuclear digestion followed by examination of specific chromatin loci indicated that ncRNAs collectively promote chromatin accessability, RNAPII-mediated interactions and overall 3D-genome organization. Our study demonstrates that RICh-PET and related methods represent a powerful suite of tools to interrogate the genome biology of ncRNAs.
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Overall design |
Investigation of RNA-chromatin Interactome by RICh-PET
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Contributor(s) |
Zheng M, Luo O, Tian S, Ruan Y |
Citation missing |
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Submission date |
Aug 17, 2016 |
Last update date |
Aug 02, 2021 |
Contact name |
Zhongyuan Tian |
E-mail(s) |
simon.tian@jax.org
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Phone |
860-837-2358
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Organization name |
The Jackson Laboratory for Genomic Medicine
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Street address |
10 Discovery Drive
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City |
Farmington |
State/province |
CT |
ZIP/Postal code |
06032 |
Country |
USA |
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Platforms (2) |
GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
GPL22339 |
Illumina NextSeq 500 (Drosophila melanogaster; Homo sapiens) |
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Samples (19)
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Relations |
BioProject |
PRJNA339288 |
SRA |
SRP082316 |