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Series GSE85575 Query DataSets for GSE85575
Status Public on Aug 13, 2016
Title An automatec microwell platform for large-scale single cell RNA-seq.
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report an automated microwell array platform for single cell RNA-seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.
Overall design Performed single cell RNA-seq on thousands of cells from three cell lines.
Contributor(s) Yuan J, Sims PA
Citation(s) 27670648
Submission date Aug 12, 2016
Last update date May 15, 2019
Contact name Peter Sims
Organization name Columbia University
Street address 3960 Broadway, 2nd Floor, Room 203AC
City New York
State/province NY
ZIP/Postal code 10032
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
Samples (2)
GSM2277951 TS543_scRNAseq
GSM2277952 U87_3T3_scRNAseq
BioProject PRJNA338815
SRA SRP081644

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85575_RAW.tar 25.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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