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Series GSE85358 Query DataSets for GSE85358
Status Public on Jan 20, 2017
Title Metabolic alterations in aged human skin in vivo
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Aging human skin undergoes significant morphological and functional changes such as wrinkle formation, reduced wound healing capacity, and altered epidermal barrier function. Besides known age-related alterations like DNA-methylation changes, metabolic adaptations have been more recently linked to impaired skin function in old humans. Understanding of these metabolic adaptations in aged skin are of special interest, because topical treatments could reverse age-dependent metabolic changes of human skin in vivo to affect age associated skin disorders.
Results: We investigated the global metabolic adaptions in human skin during aging with a combined transcriptomic and metabolomic approach applied to epidermal tissue samples of young and old human volunteers. Our analysis confirmed known age-dependent metabolic alterations, e.g. reduction of coenzyme Q10 levels, and also revealed novel age effects that are seemingly important for skin maintenance. Integration of donor-matched transcriptome and metabolome data highlighted transcriptionally-driven alterations of metabolism during aging such as altered activity in upper glycolysis and glycerolipid biosynthesis or decreased protein and polyamine biosynthesis. Together, we identified several age-dependent metabolic alterations that might affect cellular signaling, epidermal barrier function, and skin structure and morphology.
Conclusion: Our study provides a global resource on the metabolic adaptations and its transcriptional regulation during aging of human skin. Thus, it represents a first step towards an understanding of the impact of metabolism on impaired skin function in aged humans and therefore will potentially lead to improved treatments of age related skin disorders
 
Overall design Gene expression in epidermal skin samples from the inner forearms 24 young (20 to 25 years) and 24 old (55 to 66 years) human volunteers were analysed using Agilent Whole Human Genome Oligo Microarrays 8x60K V2.
 
Contributor(s) Kuehne A, Hildebrand J, Soehle J, Wenck H, Terstegen L, Gallinat S, Knott A, Winnefeld M, Zamboni N
Citation(s) 28201987
Submission date Aug 09, 2016
Last update date Jan 09, 2018
Contact name Nicola Zamboni
Organization name ETH Zurich
Department Institute for Molecular Systems Biology
Lab Zamboni Lab
Street address Auguste-Piccard-Hof 1
City Z├╝rich
State/province Zurich
ZIP/Postal code 8049
Country Switzerland
 
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (48)
GSM2265724 epidermis_D07_young_23y
GSM2265725 epidermis_D10_old_66y
GSM2265726 epidermis_D11_young_21y
Relations
BioProject PRJNA338267

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85358_RAW.tar 590.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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