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Series GSE85317 Query DataSets for GSE85317
Status Public on Mar 09, 2017
Title Analysis of genome-wide occupancy of menin, MLL1 and MLL2 in MCF-7 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We performed ChIP-seq using antibodies directed at menin, MLL1 and MLL2 in MCF-7 cells in order to assess the genome-wide presence of these proteins in MCF-7 cells.
Overall design MCF-7 cells were synchronized in phenol red-free medium containing 10% charcoal dextran-treated fetal bovine serum (CDT medium). Cells were treated with 10 nM estradiol (E2) or vehicle for 45 minutes. Menin, MLL1 and MLL2 ChIP-seq was performed after vehicle treatment. In addition, the menin cistrome was defined 45 min after treatment with E2.
Contributor(s) Dreijerink KM, Groner AC
Citation(s) 28273452
Submission date Aug 08, 2016
Last update date May 15, 2019
Contact name Koen M.A. Dreijerink
Organization name Dana-Farber Cancer Institute
Department Medical Oncology
Street address 450 Brookline Avenue
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (7)
GSM2264617 MCF7_Menin_Veh
GSM2264618 MCF7_input_Veh
GSM2264619 MCF7_Menin_E2
BioProject PRJNA338168
SRA SRP081098

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Supplementary file Size Download File type/resource
GSE85317_RAW.tar 1.2 Gb (http)(custom) TAR (of BED, BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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