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Series GSE85221 Query DataSets for GSE85221
Status Public on Feb 22, 2018
Title Developmental Exposure to 2,2’,4, 4’ –Tetrabromodiphenyl Ether Induces Long-Lasting Changes in Liver Metabolism in Male Mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Polybrominated diphenyl ethers (PBDEs) were used as flame-retardant additives in a wide range of polymers starting in 1965 and were recently withdrawn from commerce in North America and Europe. Generations that were exposed perinatally to the highest environmental doses of PBDE have now reached 5-20 years of age and in the U.S. account for 1/5 of the total population. Emerging data indicates long-term impairment of metabolic health by PBDE exposure in humans and laboratory animals. We hypothesize that exposure to PBDE during sensitive developmental windows may result in long-lasting changes in liver metabolism. In this study pregnant CD-1 mice were exposed to 0.2 mg/kg 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) from gestation day 8 till postnatal day 21 and liver RNA-seq was performed on the last day of dam exposure and on postnatal week 20 in male offspring. Several groups of metabolic genes, including ribosomal and mitochondrial genes were significantly upregulated at both time-points. Genes regulated via mechanistic target of rapamycin (mTOR pathway), the gatekeeper of metabolic homeostasis, were whether up- or down- regulated at both time-points. Thus, perinatal exposure to environmentally relevant doses of BDE-47 in laboratory mice results in long-lasting changes in liver metabolism. Our evidence suggests involvement of the mTOR pathway in the observed metabolic programming of liver.
Overall design RNA-seq analysis of liver genes in control and exposed male mice (n = 4-5/exposure) on postanatal day 21 and postnatal week 20 following perinatal exposure to BDE-47
Contributor(s) Suvorov A
Citation(s) 29264491
Submission date Aug 04, 2016
Last update date May 15, 2019
Contact name Alexander Suvorov
Organization name University of Massachusetts
Department Environmental Health Sciences
Street address 149A Goessman, 686 N.Pleasant Str
City Amherst
State/province MA
ZIP/Postal code 01003-9303
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM2262345 PND21_X90
GSM2262346 PND21_C30
GSM2262347 PND21_C19
BioProject PRJNA336571
SRA SRP080961

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Supplementary file Size Download File type/resource
GSE85221_PND21_processed.xlsx 2.9 Mb (ftp)(http) XLSX
GSE85221_PNW20_processed.xlsx 1.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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