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Series GSE85170 Query DataSets for GSE85170
Status Public on Apr 01, 2018
Title MEK inhibition rewires enhancer landscapes in RAS-driven Rhabdomyosarcoma to unlock a myogenic differentation block
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Trametinib-treated rhabdomyosarcoma cells undergo transcriptional reprogramming akin to myogenic differentiation. This reprogramming is induced by loss of ERK-mediated inhibition of MYOG expression. Restoration of MYOG allows establishment of super-enhancers at genes expressed by terminally differentiated myotubes. Our findings demonstrate that aberrant MAP kinase activity blocks differentiation in rhabdomyosarcoma and highlight trametinib as a potential therapeutic for RAS-mutated rhabdomyosarcoma.
Overall design RNA-seq in 2 FN-RMS cell lines exposed to either DMSO or Trametinib treatment for various concentrations and timepoints, and siRNA against MEK1 or scramble.
Contributor(s) Gryder BE, Yohe ME, Wen X
Citation(s) 29973406
Submission date Aug 03, 2016
Last update date May 15, 2019
Contact name Javed Khan
Phone 2407606135
Organization name NCI, NIH
Department Genetics Branch
Lab Javed Khan
Street address 37 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM2259159 CTR untreated
GSM2259161 CTR 10 nM Tram, 6 hrs
This SubSeries is part of SuperSeries:
GSE85171 Epigenetic Reprogramming of mutant RAS-driven Rhabdomyosarcoma via MEK Inhibition
BioProject PRJNA336381
SRA SRP080882

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85170_RAW.tar 14.0 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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