GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE85111 Query DataSets for GSE85111
Status Public on May 17, 2017
Title inDrop single cell RNA-seq of hematopoietic cells derived from human pluripotent stem cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed morphogen-directed differentiation of human PSCs into HE followed by combinatorial screening of 26 candidate HSC-specifying TFs for the potential to promote hematopoietic engraftment in irradiated immune deficient murine hosts. We recovered seven TFs (ERG, HOXA5, HOXA9, HOXA10, LCOR, RUNX1, SPI1) that together were sufficient to convert HE into hematopoietic stem and progenitor cells (HSPCs) that engraft primary and secondary murine recipients
Overall design Examination of expression pattern in hematopoietic cells.
Contributor(s) Sugimura R, Daley G
Citation(s) 28514439
Submission date Aug 02, 2016
Last update date May 15, 2019
Contact name Areum Han
Organization name Boston Children's Hospital
Lab Daley lab
Street address Karp Family Building, 7th Floor. 300 Longwood Ave.
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM2257877 HSPC from hiPSC_RS1 (single-cell RNA-seq)
GSM2257878 HSPC from CB_RS2 (single-cell RNA-seq)
This SubSeries is part of SuperSeries:
GSE85112 Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells via Transcription Factor Conversion of Hemogenic Endothelium
BioProject PRJNA336240
SRA SRP080883

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85111_RAW.tar 45.4 Mb (http)(custom) TAR (of TXT)
GSE85111_README.txt 1017 b (ftp)(http) TXT
GSE85111_gel_barcode1_list.txt.gz 1.4 Kb (ftp)(http) TXT
GSE85111_gel_barcode2_list.txt.gz 1.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap