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Series GSE85089 Query DataSets for GSE85089
Status Public on Oct 08, 2017
Title Integrator orchestrates RAS/ERK1/2 signaling transcriptional programs
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary We investigated the occupancy of RNA PolII and INTS11 during the stimulation of EGF and compared with drug treated condition using inhibitors against MAPK pathway and Integrator. Additionally, we examined transcription by sequencing the chromatin-bound fraction of RNA.
Overall design We employed several cel lines in our experiment, including HELA, KRAS mutant lung cancer cell line A549 and BRAF mutatant melanoma cell line A375. The conditons we checked including EGF stimulation, MAPK pathway inhibition using BRAF, MEK or ERK inhibitiors, targeting INTS11 with RNAi or integrator inhibitor. We used RNA sequencing to measure the expression profile and CHIP sequencing to detect INTS11 and RNA PolII recruitment on chromatin.
Web link
Contributor(s) Yue J, Lai F, Pastori C, Zhang A, Beckedorff F, Shiekhattar R
Citation(s) 28982763
Submission date Aug 02, 2016
Last update date Jul 25, 2021
Contact name Jingyin Yue
Phone 305-243-7394
Organization name University of Miami
Department Sylvester Comprehensive Cancer Center
Lab Shiekhattar, Ramin
Street address 1501 NW 10th Ave
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (58)
GSM2257539 HELA cell RNA-seq with vehicle treatment
GSM2257540 HELA cell RNA-seq with vehicle treatment plus EGF stimulation
GSM2257541 HELA cell RNA-seq after INTS11 knockdown
BioProject PRJNA336122
SRA SRP080797

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85089_RAW.tar 84.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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