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Series GSE85082 Query DataSets for GSE85082
Status Public on Nov 28, 2017
Title Control of gene expression in senescence through transcriptional read-through of convergent protein-coding genes [RNA-Seq PROLIF-SEN]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Antisense RNAs are non-coding RNAs, which can regulate their corresponding sense RNAs and are generally produced from specific promoters. By genome-wide approaches and in depth analyses at specific loci in human cells undergoing senescence, we uncover a family of antisense RNAs produced by transcriptional read-through at convergent protein-coding genes. Importantly, these antisense RNAs, that we named STARTs, repress the expression of their corresponding sense RNAs. We found that the elongation rate of RNA pol II is limited downstream of the TTS at START loci in proliferative cells. This allows transcription termination to occur before the RNA pol II reaches the convergent genes, thus preventing antisense RNA production and interference with the expression of the convergent genes. In proliferative cells, STARTs are repressed by the histone variant H2A.Z, whose local occupancy decreases in senescence. Our results thus uncover a novel mechanism of gene expression regulation, relying on the control of the expression of read-through antisense transcripts at convergent genes and underline the functional importance of the epigenetic control of RNA pol II elongation rate at intergenic regions.
Overall design Strand-specific RNA-seq datasets in proliferative cells and in Raf1-induced senescent cells (2 replicates).
Contributor(s) Lazorthes S, Muniz L, Aguirrebengoa M, Trouche D, Nicolas E
Citation(s) 29186682
Submission date Aug 02, 2016
Last update date Jul 25, 2021
Contact name Estelle Nicolas
Phone +33 5 61 55 81 11
Organization name INSERM
Lab UMR5077, MCD, CBI
Street address 118 route de Narbonne
City Toulouse
ZIP/Postal code 31062
Country France
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM2781951 Strand-specific RNA-seq in proliferative cells- Replicate #1
GSM2781952 Strand-specific RNA-seq in senescent cells- Replicate #1
GSM2781953 Strand-specific RNA-seq in proliferative cells- Replicate #2
This SubSeries is part of SuperSeries:
GSE85085 Control of gene expression in senescence through transcriptional read-through of convergent protein-coding genes
BioProject PRJNA336104
SRA SRP080789

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Supplementary file Size Download File type/resource
GSE85082_RAW.tar 1.6 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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