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Series GSE85071 Query DataSets for GSE85071
Status Public on Sep 02, 2016
Title The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan (ATAC-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Neutrophils, eosinophils and “classical” monocytes collectively account for ~70% of human blood leukocytes and are among the shortest-lived cells in the body. Precise regulation of the lifespan of these myeloid cells is critical to maintain protective immune responses while minimizing the deleterious consequences of prolonged inflammation. However, how the lifespan of these cells is strictly controlled remains largely unknown. Here, we identify a novel long non-coding RNA (lncRNA) that we termed Morrbid, which tightly controls the survival of neutrophils, eosinophils and “classical” monocytes in response to pro-survival cytokines. To control the lifespan of these cells, Morrbid regulates the transcription of its neighboring pro-apoptotic gene, Bcl2l11 (Bim), by promoting the enrichment of the PRC2 complex at the Bcl2l11 promoter to maintain this gene in a poised state. Notably, Morrbid regulates this process in cis, enabling allele-specific control of Bcl2l11 transcription. Thus, in these highly inflammatory cells, changes in Morrbid levels provide a locus-specific regulatory mechanism that allows for rapid control of apoptosis in response to extracellular pro-survival signals. As MORRBID is also present in humans and dysregulated in patients with hypereosinophilic syndrome, this lncRNA may represent a potential therapeutic target for inflammatory disorders characterized by aberrant short-lived myeloid cell lifespan.
Overall design Examination of chromatin accessibility in mouse neutrophils.
Contributor(s) Kotzin JJ, Spencer SP, McCright SJ, Uthaya Kumar DB, Collet M, Mowel WK, Elliott EN, Uyar A, Makiya MA, Dunagin MC, Harman CD, Virtue AT, Zhu S, Bailis W, Stein J, Hughes C, Raj A, Wherry EJ, Goff LA, Klion AD, Rinn JL, Williams A, Flavell RA, Henao-Mejia J
Citation(s) 27525555
Submission date Aug 01, 2016
Last update date May 15, 2019
Contact name Asli Uyar
Organization name The Jackson Laboratory
Department Computational Sciences
Street address 10 Discovery Dr
City Farmington
State/province CT
ZIP/Postal code 06032
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2257327 KO_1 ATAC-Seq
GSM2257328 KO_2 ATAC-Seq
GSM2257329 WT_1 ATAC-Seq
This SubSeries is part of SuperSeries:
GSE85073 The long non-coding RNA Morrbid regulates Bim and short-lived myeloid cell lifespan
BioProject PRJNA336055
SRA SRP080771

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Supplementary file Size Download File type/resource
GSE85071_ATAC_Seq_consensusPeaks_KO_and_WT.xls.gz 1.7 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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