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Series GSE85062 Query DataSets for GSE85062
Status Public on May 24, 2017
Title Enforcement of developmental lineage specificity by transcription factor Oct1 (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Embryonic stem cells (ESCs) co-express Oct4/POU5F1 and an Oct4 paralog known Oct1/POU2F1. To study the role of Oct1 in embryonic stem cell transcriptional control and pluripotency, we constructed germline and inducible-conditional Oct1 deficient ESC lines. ESCs lacking Oct1 show normal appearance, self-renewal, growth rates and metabolic signatures. However loss of Oct1 results in defective lineage specification. ESCs lacking Oct1 fail to form beating cardiomyocytes in culture, generate neurons poorly, form smaller, less differentiated teratomas, and are incapable of generating chimeric mice. Upon RA-mediated neuronal differentiation, Oct1 deficient cells fail to properly induce developmentally poised genes. Simultaneously, genes for alternative developmental pathways, most notably for the development of extra-embryonic tissues, are inappropriately expressed. ChIP experiments show that Oct1 does not occupy pluripotency genes or differentially expressed developmental targets in ESCs. Additionally, Oct1 occupies developmental targets as cells differentiate and Oct4 is lost. These results are consistent with a role for Oct1 in promoting the expression of lineage-specific genes in differentiating cells while simultaneously repressing genes specific for alternative lineages.
Overall design Examination of occupancy of Oct1 and Oct4 in mouse embryonic stem cells
Contributor(s) Shen Z, Shakya A, Kang J, Tabaka M, Jarboe E, Regev A, Tantin D
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 01, 2016
Last update date May 15, 2019
Contact name Brett Milash
Organization name University of Utah
Department Center for High Performance Computing
Street address 155 S. 1452 E. Room 414
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM2257272 Oct1 ChIPseq
GSM2257273 Oct4 ChIPseq
GSM2257274 H3K4me3 ChIPseq
This SubSeries is part of SuperSeries:
GSE85063 Enforcement of developmental lineage specificity by transcription factor Oct1
BioProject PRJNA336049
SRA SRP080766

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Supplementary file Size Download File type/resource
GSE85062_RAW.tar 760.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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