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Series GSE85057 Query DataSets for GSE85057
Status Public on Oct 11, 2016
Title HMGB2 orchestrates the chromatin landscape of senescence-associated secretory phenotype gene loci.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Cellular senescence is a stable cell growth arrest that is characterized by silencing of proliferation-promoting genes through compaction of individual chromosomes into senescence-associated heterochromatin foci (SAHF). Paradoxically, senescence is also accompanied by increased expression of certain secreted factors such as cytokines and chemokines, known as senescence-associated secretory phenotype (SASP). How SASP genes are excluded from SAHF-mediated global gene silencing remains unclear. Here we report that HMGB2 orchestrates the chromatin landscape of SASP gene loci. HMGB2 preferentially localizes to SASP gene loci during senescence. Loss of HMGB2 during senescence blunts SASP gene expression by allowing for spreading of repressive heterochromatin into SASP gene loci. This correlated with incorporation of SASP gene loci into SAHF. Our results establish HMGB2 as a novel master regulator that orchestrates SASP through preventing heterochromatin spreading to allow for exclusion of SASP gene loci from a global heterochromatin environment during senescence.
Overall design HMGB2 CHIP-seq in senescent and growing IMR90 cells
Contributor(s) Aird KM, Zhang R
Citation(s) 27799366
Submission date Aug 01, 2016
Last update date May 15, 2019
Contact name Priyankara J Wickramasinghe
Phone 2154956837
Organization name The Wistar Institute
Department Bioinformatics
Lab Genomics
Street address 3601 Spruce Street
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (2)
GSM2257241 HMGB2 CHIP-seq in senescent cells
GSM2257242 HMGB2 CHIP-seq in growing cells
BioProject PRJNA336027
SRA SRP080758

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Supplementary file Size Download File type/resource
GSE85057_peaks_SvsGR_broad.bed.txt.gz 4.8 Kb (ftp)(http) TXT
GSE85057_peaks_SvsGR_narrow.bed.txt.gz 35.5 Kb (ftp)(http) TXT
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