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Series GSE85027 Query DataSets for GSE85027
Status Public on Mar 16, 2017
Title Dido as a switchboard that regulates self-renewal and differentiation in embryonic stem cells (vsmutNT)
Organism Mus musculus
Experiment type Expression profiling by array
Summary Transition from symmetric to asymmetric cell division requires precise coordination of differential gene expression. Embryonic stem cells (ESC) strongly express Dido3, whose C-terminal truncation impedes ESC differentiation while retaining self-renewal. We show that Dido3 binds to its gene locus via H3K4me3 and RNA pol II and, at differentiation onset, induces expression of its splice variant Dido1, which then leads to Dido3 degradation and downregulation of stemness genes. We propose that Dido isoforms act as a switchboard to regulate genetic programs for ESC transition from pluripotency maintenance to promotion of differentiation.
We used microarrays to determine how the deletion of exon 16 (C-terminal truncation) of the Dido3 affects gene expression that results in the suppression of ESC differentiation.
Overall design Mouse embryoid bodies from 2 conditions, wild type and truncated C-terminal Dido3 protein mutant overexpressing the N-terminal common region containing the PHD domain of the Dido gene, were cultured in pararell and equal conditions. After 10 days development, RNA was extracted and processed for hybridization on Agilent microarrays (Agilent Mouse GE 4x44K v2 Microarray). Three biological replicates for each condition were processed simultaneously.
Contributor(s) Futterer A, De-Celis J
Citation(s) 28330622
Submission date Aug 01, 2016
Last update date Jan 19, 2018
Contact name Carlos Martinez
Organization name Centro Nacional de Biotecnologia
Department Inmunology and Oncology
Lab 411
Street address C/Darwin 3
City Madrid
State/province Madrid
ZIP/Postal code 28043
Country Spain
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (3)
GSM2256421 10 days mouse embryoid body NT, biological rep1
GSM2256422 10 days mouse embryoid body NT, biological rep2
GSM2256423 10 days mouse embryoid body NT, biological rep3
This SubSeries is part of SuperSeries:
GSE85029 Dido as a switchboard that regulates self-renewal and differentiation in embryonic stem cells
BioProject PRJNA335993

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85027_RAW.tar 52.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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