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Series GSE85019 Query DataSets for GSE85019
Status Public on Sep 30, 2017
Title CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Through RNA-seq of wildtype and CFP1-deleted GV oocytes of different ages, zygotes and 2-cell embryos and whole genome bisulfite sequencing of GV oocytes, We show here that CFP1 is responsible for epigenetic maturation in oocytes. Deletion of CFP1 directly decreased histone H3K4 trimethylation and caused global down-regulation of gene expression in oocytes.These genes are involved in cytoplasmic lattice formation, maternal-zygotic transition and epigenetic maturation. Maternal CFP1-deleted oocytes had fewer CPLs in the cytoplasm and the organelles were severely aggregated, which further caused defects in α-tubulin polymerization and aneuploidy in meiosis II. The genome was less methylated and methylation of maternal DNA was impaired after CFP1 deletion. Therefore CFP1-deleted oocytes fail to complete epigenetic maturation as well as cytoplasmic maturation and nuclear maturation and unable to gain developmental competence during oogenesis.
Overall design GV oocyte, zygote and 2-cell embryonic transcriptome profiles wild type (WT) and CFP1-depleted mice were generated by deep sequencing, in duplicate or triplicate on Illumina platform.
Contributor(s) Yu C, Fan X
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Submission date Jul 31, 2016
Last update date May 15, 2019
Contact name Xiaoying Fan
Organization name Peking University
Department College of Life Sciences
Lab Biodynamic Optical Imaging Center
Street address 5 Yiheyuan Road
City Beijing
State/province Beijing
ZIP/Postal code 100871
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (23)
GSM2256355 GV_WT_1
GSM2256356 GV_WT_2
GSM2256357 GV_WT_3
BioProject PRJNA335914
SRA SRP080711

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Supplementary file Size Download File type/resource
GSE85019_sample_fpkm.txt.gz 1.4 Mb (ftp)(http) TXT
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Processed data are available on Series record
Processed data provided as supplementary file
Raw data are available in SRA

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