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Series GSE85 Query DataSets for GSE85
Status Public on Sep 19, 2002
Title wild type and aire -/- murine meduallary thymic epithelial cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Keywords: ordered
Citation(s) 12376594
Submission date Sep 18, 2002
Last update date Feb 18, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platforms (1)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
Samples (6)
GSM2444 B7hi mTEC B6/129 wt1
GSM2445 B7hi mTEC B6/129 wt2
GSM2446 B7hi mTEC B6/129 wt3
BioProject PRJNA85463

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Supplementary file Size Download File type/resource
GSE85_RAW.tar 14.9 Mb (http)(custom) TAR (of CEL)

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