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Series GSE84938 Query DataSets for GSE84938
Status Public on Jun 10, 2017
Title Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line [miRNA expression]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Transcriptional profiling and microRNA profiling of paired PDX and derived cell line MT-CHC01 upon ET-743 treatement
Intrahepatic cholangiocarcinoma (ICC) is an aggressive and lethal malignancy with limited therapeutic options. ET-743 has a high antitumor activity in preclinical models of biliary tract carcinoma (BTC), being a promising alternative treatment. Here, we studied the effect of ET-743 at transcriptomic level on an ICC patient derived xenograft (PDX) and on the derived cell line, MT-CHC01. Further, putative targets of ET-743 were explored in the in vitro model. In vitro, ET-743 inhibited genes involved in protein modification, neurogenesis, migration, and motility; it induced the expression of genes involved in keratinization, tissues development, and apoptotic processes. In the PDX model, ET-743 affected ECM-receptor interaction, focal adhesion, complement and coagulation cascades, Hedgehog, MAPK, EGFR signaling via PIP3 pathway, and apoptosis. In MT-CHC01, 24 microRNAs were deregulated upon drug treatment. Only 5 microRNAs were perturbed by ET-743 in PDX; 2 up and 3 down-regulated. Among down- regulated genes, we selected SYK and LGALS1; their silencing caused a significantly reduction of migration, but did not affect proliferation in MT-CHC01 and WITT cells. In conclusion, we described that ET-743 affected genes and microRNAs involved in tumor progression and metastatic processes, reflecting data previously obtained at macroscopically level; in particular, we identified SYK and LGALS1 as new putative targets of ET-743.
Overall design MicroRNA alterations upon ET-743 treatement
MT-CHC01 cell line treated with ET-743 at the dose of 5 nM for 24 hours vs MT-CHC01 cell line untreated;ICC PDX treated with ET-743 at the dose of 0.15 mg/Kg/weekly for three weeks vs ICC PDX untreated
Contributor(s) Neia CP, cavalloni G, Chiorino G, Ostano P, Leone F, Aglietta M
Citation(s) 27902465
Submission date Jul 28, 2016
Last update date Jun 10, 2017
Contact name giovanna chiorino
Organization name Fondo Edo Tempia
Department Cancer Genomics
Street address via malta 3
City Biella
ZIP/Postal code 13900
Country Italy
Platforms (1)
GPL15159 Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray 030840 (Probe Name version)
Samples (8)
GSM2254421 MT-CHC01-NT1 [miRNA]
GSM2254422 MT-CHC01-NT2 [miRNA]
GSM2254423 MT-CHC01-ET-743-1 [miRNA]
This SubSeries is part of SuperSeries:
GSE84939 Gene and microRNA modulation upon ET-743 treatment in a human intrahepatic cholangiocarcinoma paired patient derived xenograft and cell line
BioProject PRJNA335715

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84938_RAW.tar 62.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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