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Series GSE84899 Query DataSets for GSE84899
Status Public on Dec 20, 2016
Title Cdx and T Brachyury co-activate growth signaling in the embryonic axial progenitor niche.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary In vertebrate embryos, anterior tissues are generated early, followed by the other axial structures that emerge sequentially from a posterior growth zone. The genetic network driving posterior axial elongation in mice, and its disturbance in mutants with posterior truncation are not yet fully understood. We show that the combined expression of Cdx2 and T Brachyury is essential to establish the core signature of posterior axial progenitors. Cdx2 and T Brachyury are required for extension of a similar trunk portion of the axis. Simultaneous loss of function of these two genes disrupts axial elongation to a much greater extent than each single mutation alone. We identify and validate common targets for Cdx2 and T Brachyury in vivo including Wnt and Fgf pathway components active in the axial progenitor niche. Our data demonstrate that integration of the Cdx/Hox and T Brachyury transcriptional networks controls differential axial growth during vertebrate trunk elongation.
Overall design ChIP-seq for several transcription factors and histone mark H3K27ac, ATAC-seq and RNA-seq in Epiblast stem cells (EpiSCs) and early stage embryos.
Contributor(s) Amin S, Neijts R, Simmini S, van Rooijen C, Tan SC, Kester L, Oudenaarden V, Creyghton MP, Deschamps J
Citation(s) 28009287
Submission date Jul 27, 2016
Last update date May 15, 2019
Contact name Jacqueline Deschamps
Organization name Hubrecht Institute
Street address Uppsalalaan 8
City Utrecht
ZIP/Postal code 3584CT
Country Netherlands
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (55)
GSM2253706 CBP-P300 WTEpiSCs 24h rep1
GSM2253707 Cdx2 wtEpiSCs 24h rep1
GSM2253708 Cdx2 wtEpiSCs 24h rep2
BioProject PRJNA335589
SRA SRP079971

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84899_Amin_CEL-Seq_CellLines.csv.gz 250.1 Kb (ftp)(http) CSV
GSE84899_Amin_CEL-Seq_Embryos.csv.gz 217.2 Kb (ftp)(http) CSV
GSE84899_RAW.tar 9.3 Gb (http)(custom) TAR (of BED, BW, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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