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Series GSE84872 Query DataSets for GSE84872
Status Public on Jul 26, 2016
Title Gene-regulatory networks activated by pattern-specific generation of action potentials in dorsal root ganglia neurons
Organism Mus musculus
Experiment type Expression profiling by array
Summary Development and plasticity in the nervous system are regulated by specific patterns of neural impulse activity. Patterned activity orchestrates spatial and temporal changes in the expression of networks of genes. These gene regulatory networks underlie the long-term changes in cell specification, growth of synaptic connections, and frequency adaptation that occur throughout neonatal and postnatal life. We show that the temporal nature of action potentials and downstream signaling differentially regulate the expression of hundreds of diverse neuronal genes through distinct gene regulatory networks. Analysis of upstream regulatory regions showed enrichment in transcription factor binding sites for NF-Kappa that was action potential firing pattern specific. Our new findings demonstrate spike-frequency decoding by action potentials at the transcriptional level by pattern-specific activation of transcription factors and associated gene-regulatory networks that operate to adjust the modalities of sensory neurons.
 
Overall design We analyzed the transcriptomic changes in primary cultures of embronic dorsal root ganglia (DRG) neurons subjected to four electric stimulation patterns with respect to their non-stimulated counterparts.
We have adopted the multiple yellow strategy in which differently fluorescent labeled biological replicas are co-hybridized with each array and then similarly labeled samples from cells subjected to different conditions are compared and results averaged over the two labels.
 
Contributor(s) Lee PR, Cohen JE, Iacobas DA, Iacobas S, Fields RD
Citation(s) 28256583, 31561430
Submission date Jul 26, 2016
Last update date Nov 25, 2019
Contact name Dumitru Andrei Iacobas
E-mail(s) daiacobas@pvamu.edu
Phone 936-261-9926
Organization name Prairie View A&M University
Department Center for Computational Systems Biology
Lab Personalized Genomics
Street address Ann Preston St
City Prairie View
State/province TX
ZIP/Postal code 77446
Country USA
 
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (20)
GSM2252922 X1
GSM2252923 X2
GSM2252924 X3
Relations
BioProject PRJNA335407

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84872_A1A2_US83000181_252665510921_S01_GE2_107_Sep09_1_4.txt.gz 15.4 Mb (ftp)(http) TXT
GSE84872_A3A4_US83000181_252665510921_S01_GE2_107_Sep09_1_3.txt.gz 15.4 Mb (ftp)(http) TXT
GSE84872_B1B3_US83000181_252665510920_S01_GE2_107_Sep09_1_2.txt.gz 15.5 Mb (ftp)(http) TXT
GSE84872_B2B4_US83000181_252665510920_S01_GE2_107_Sep09_1_1.txt.gz 15.5 Mb (ftp)(http) TXT
GSE84872_C1C3_US83000181_252665510920_S01_GE2_107_Sep09_1_4.txt.gz 15.3 Mb (ftp)(http) TXT
GSE84872_C2C4_US83000181_252665510920_S01_GE2_107_Sep09_1_3.txt.gz 15.4 Mb (ftp)(http) TXT
GSE84872_D1D2_US83000181_252665510919_S01_GE2_107_Sep09_1_4.txt.gz 15.3 Mb (ftp)(http) TXT
GSE84872_D3D4_US83000181_252665510919_S01_GE2_107_Sep09_1_3.txt.gz 15.3 Mb (ftp)(http) TXT
GSE84872_RAW.tar 6.5 Mb (http)(custom) TAR
GSE84872_X1X2_US83000181_252665510921_S01_GE2_107_Sep09_1_2.txt.gz 15.3 Mb (ftp)(http) TXT
GSE84872_X3X4_US83000181_252665510921_S01_GE2_107_Sep09_1_1.txt.gz 15.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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