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Series GSE84706 Query DataSets for GSE84706
Status Public on Jul 10, 2017
Title Experimental validation of candidate regulators of KRAS transcriptional components.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We sequenced mRNA from HCC44 and YAPC cell lines stably expressing Cas9, and infecting with indicated gRNAs against regulators of KRAS transcriptional components.
Overall design KRAS mutant cell lines HCC44 and YAPC stably expressing with Cas9 were infected with three independent gRNAs targeting indicated genes or controls (GFP, RFP, random gRNAs) and harvested 8 days after infection.
Contributor(s) Kim JW, Mesirov J, Hahn WC, Tamayo P
Citation(s) 28837809
Submission date Jul 21, 2016
Last update date May 15, 2019
Contact name Jong Wook Kim
Organization name Broad Institute
Department Cancer Program
Street address 45 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (21)
GSM2248003 HCC44_ZEB1_1
GSM2248004 HCC44_ZEB1_2
GSM2248005 HCC44_ZEB1_3
BioProject PRJNA330865
SRA SRP079257

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Supplementary file Size Download File type/resource
GSE84706_2_RNAseq_CRISPR.rpkm.v2.gct.gz 3.0 Mb (ftp)(http) GCT
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Raw data are available in SRA
Processed data are available on Series record

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