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Series GSE84686 Query DataSets for GSE84686
Status Public on Aug 09, 2017
Title Innate to adaptive: Human IFN-gamma producing CD4+ T cells can derive directly from CXCL8-producing recent thymic emigrants.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary An unanticipated feature of the human neonatal CD4 T cell response is a robust capacity to produce CXCL8. However, this 'innate-like' function dissipates with age and is scarce in the adult. Here, we investigated the fate of CD4+CXCL8+ cells and their transition into conventional adaptive T cells. We show that CXCL8 is imprinted on immature thymocytes prior to TCR signalling and is maintained in T cell committed thymic progenitors and recent thymic emigrants (RTEs) of adults as well as neonates. Hence, rather than being unique to neonates, CXCL8-producing CD4+ T cells decrease with age in humans (and in humanised mice) owing to the decline in thymic output, coupled with the cells’ peripheral expansion. By cloning of CXCL8+CD4+ cells from cord blood, we were able to track effector function within daughter cells and demonstrate that these cells can convert to IFN-g producing cells. In sum, we provide direct evidence that ‘innate like’ CXCL8-producing CD4+ T cells emerge from the thymus and can transition into conventional adaptive Th1 cells
Overall design Examination of RNA-Seq count data from 96 single cells
Contributor(s) Das A, Rouault-Pierre K, Gomez-Tourino I, Wood K, Donaldson IM, Mein CA, Bonnet D, Hayday AC, Gibbons DL
Citation(s) 28754679
Submission date Jul 21, 2016
Last update date May 15, 2019
Contact name Deena L. Gibbons
Phone +44 020 7188 0150
Organization name Kings College London
Department Dept. Immunology
Street address Great Maze Pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (96)
GSM2247585 Chip2-A10_S13
GSM2247586 Chip2-A1_S1
GSM2247587 Chip2-A12_S25
BioProject PRJNA330840
SRA SRP079213

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Supplementary file Size Download File type/resource
GSE84686_consolidated_raw_counts_final.txt.gz 625.5 Kb (ftp)(http) TXT
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