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Series GSE84683 Query DataSets for GSE84683
Status Public on Nov 06, 2019
Title Differential expression analysis of RNA-seq data from melanocytes driven by tumor cell-derived exosomes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Exosomes (40–100 nm) are organelle-like membranous structures shed into interstitial spaces and body fluids under diverse pathophysiologic conditions, including tumor development. The tumor microenvironment is abundant with exosomes secreted by the cancer cells themselves. Studies have shown that tumor-derived exosomes can transport RNA and active molecules to other cells to promote tumor growth. Exosomes are increasingly being recognized as a major contributor in the progression of malignant neoplasms. We have found that normal melanocytes can acquire invasiveness through the internalization of melanoma cell-derived exosomes. However, little is known about how the exosomes modulate the melanocytes in the microenvironment to optimize conditions for tumor progression and metastasis. We hypothesize that melanoma cell-derived exosomes can drive the dysregulation of transcriptomes in normal melanocytes and facilitate melanoma progression. Our objective is to identify differentially expressed genes in melanocytes driven by tumor cell-derived exosomes through RNA sequencing and translate those genes as therapeutic targets for melanoma metastasis.
Overall design Total RNA was extracted from three groups of human melanocytes: NHEM-c melanocytes without exosomes (control group), NHEM-c melanocytes co-cultured with A375 melanoma cell-derived exosomes, and NHEM-c melanocytes co-cultured with A549 lung cancer cellderived exosomes.
Contributor(s) Xiao D, Qu J, Hao H, Rouchka EC, Li X, McMasters K
Citation(s) 31678172
Submission date Jul 21, 2016
Last update date Nov 07, 2019
Contact name Hongying Hao
Phone 502-852-8404
Organization name University of Louisville
Department Surgery
Lab Dr. Kelly M McMasters
Street address 505 South Hancock Street
City Louisville
State/province KY
ZIP/Postal code 40202
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (9)
GSM2247542 NHEM-c_without_exosomes_control_rep1
GSM2247543 NHEM-c_without_exosomes_control_rep2
GSM2247544 NHEM-c_without_exosomes_control_rep3
BioProject PRJNA330826
SRA SRP079185

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Supplementary file Size Download File type/resource
GSE84683_FPKM_geneMatrix_NORMALIZED.txt.gz 1.2 Mb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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