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Series GSE84675 Query DataSets for GSE84675
Status Public on Mar 06, 2017
Title Effect of BCL11B overexpression on transcriptome of T-cell acute lymphoblastic leukemia (T-ALL) cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the effects of BCL11B on T-cell differentiation, we performed gain of function studies in cells with a T-lineage differentiation arrest, namely T-ALL cells. Gene expression profiling by RNA-Seq demonstrated that BCL11B overexpression induced transcriptional changes consistent with T-cell differentiation as early as 72 hours after transduction, indicating a rapid regulatory effect of BCL11B on the T-lineage transcriptional program and supporting an important role for BCL11B in human T-cell differentiation.
Overall design T-ALL cells were transduced with a BCL11B-GFP expression vector (overexpressing cells) or an empty GFP vector (control cells). GFP+ cells were isolated by fluorescence activation cell sorting (FACS) at 72 hours post transduction and analyzed by RNA-Seq to determine the effect of BCL11B on the transcriptome of T-ALL cells.
Contributor(s) Parekh C
Citation(s) 28232744
Submission date Jul 21, 2016
Last update date May 15, 2019
Contact name chintan parekh
Organization name Children's Hospital Los Angeles
Department Pediatrics
Street address 4650 sunset blvd, mail stop 54
City Los Angeles
State/province California
ZIP/Postal code 90027
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (6)
GSM2247484 BCL11B replicate 1
GSM2247485 Control replicate 1
GSM2247486 BCL11B replicate 2
This SubSeries is part of SuperSeries:
GSE84678 BCL11B
BioProject PRJNA330819
SRA SRP079175

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Supplementary file Size Download File type/resource
GSE84675_read_counts.txt.gz 315.9 Kb (ftp)(http) TXT
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