|
| Status |
Public on Dec 20, 2016 |
| Title |
ChIP-seq of Active Histone Mark (H3K27ac) and Myogenic Regulatory Factors (MYF5 and MYOD) in Fusion Negative Rhabdomyosarcoma Cell Lines |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed Chip-seq analysis of Myogenic Regulatory Transcription Factors (MYF5 and MYOD) in Fusion Negative Rhabdomyosarcoma cell lines. Endogenous MYF5/H3K27ac Chip-seq was performed in Rh18 cells and MYOD-H3K27ac Chip-seq was performed in RD cells, given that these cell lines express these proteins in a mutually-exclusive manner. Analysis revealed a common subset of enhancer and promoter regions bound by these transcription factors that are enriched for cell cycle regulation and embryonic muscle development pathways. Keywords: rhabdomyiosarcoma, Chip-seq, chromatin, Transcription factos, MYF5, MYOD
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| |
|
| Overall design |
5 samples are deposited including input DNA controls for each of 2 cell lines.
|
| |
|
| Contributor(s) |
Gryder BE, Yohe ME, Wen X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 20, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Nitin Roper |
| E-mail(s) |
nitin.roper@nih.gov
|
| Phone |
2408583571
|
| Organization name |
NCI, NIH
|
| Department |
Developmental Therapeutics Branch
|
| Street address |
37 Convent Dr.
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (5)
|
| GSM2243113 |
RD Fusion Negative Rhabdomyosarcoma cell line H3K27ac |
| GSM2243114 |
RD Fusion Negative Rhabdomyosarcoma cell line input |
| GSM2243115 |
RH18 H3K27ac |
|
| This SubSeries is part of SuperSeries: |
| GSE84630 |
Fusion Negative Rhabdomyosarcoma Cell Lines |
|
| Relations |
| BioProject |
PRJNA330687 |
| SRA |
SRP078996 |
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