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Series GSE84628 Query DataSets for GSE84628
Status Public on Dec 20, 2016
Title ChIP-seq of Active Histone Mark (H3K27ac) and Myogenic Regulatory Factors (MYF5 and MYOD) in Fusion Negative Rhabdomyosarcoma Cell Lines
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We performed Chip-seq analysis of Myogenic Regulatory Transcription Factors (MYF5 and MYOD) in Fusion Negative Rhabdomyosarcoma cell lines. Endogenous MYF5/H3K27ac Chip-seq was performed in Rh18 cells and MYOD-H3K27ac Chip-seq was performed in RD cells, given that these cell lines express these proteins in a mutually-exclusive manner. Analysis revealed a common subset of enhancer and promoter regions bound by these transcription factors that are enriched for cell cycle regulation and embryonic muscle development pathways. Keywords: rhabdomyiosarcoma, Chip-seq, chromatin, Transcription factos, MYF5, MYOD
Overall design 5 samples are deposited including input DNA controls for each of 2 cell lines.
Contributor(s) Gryder BE, Yohe ME, Wen X
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Submission date Jul 20, 2016
Last update date May 15, 2019
Contact name Nitin Roper
Phone 2408583571
Organization name NCI, NIH
Department Developmental Therapeutics Branch
Street address 37 Convent Dr.
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (5)
GSM2243113 RD Fusion Negative Rhabdomyosarcoma cell line H3K27ac
GSM2243114 RD Fusion Negative Rhabdomyosarcoma cell line input
GSM2243115 RH18 H3K27ac
This SubSeries is part of SuperSeries:
GSE84630 Fusion Negative Rhabdomyosarcoma Cell Lines
BioProject PRJNA330687
SRA SRP078996

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84628_RAW.tar 2.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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