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Series GSE84540 Query DataSets for GSE84540
Status Public on Nov 07, 2016
Title New transgenic mouse lines for selectively targeting astrocytes and for studying calcium signals in astrocyte processes in situ and in vivo
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The Khakh laboratory developed and characterized a new Aldh1l1-Cre/ERT2 transgenic mouse line to selectively target astrocytes in vivo. Crosses with RiboTag mice allowed sequencing of actively translated mRNAs and analyses of the cortical astrocyte transcriptome from adults.
Overall design Cortices were extraced from 4 young adult (~P80) male and female Aldh1l1-Cre/ERT2 x Ribotag mice. After homogenization, RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag.
Contributor(s) Lu T, Srinivasan R, Chai H, Xu J, Huang B, Coppola G, Golshani P, Khakh BS
Citation(s) 27939582
Submission date Jul 18, 2016
Last update date May 15, 2019
Contact name Giovanni Coppola
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM2241695 579 input
GSM2241696 579 IP
GSM2241697 580 input
BioProject PRJNA329559
SRA SRP078880

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84540_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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